A Theoretical and Experimental Study for Enzymatic Biodiesel Production from Babassu Oil (Orbignya sp.) Using Eversa Lipase

A theoretical and experimental study was carried out on the biocatalytic production of babassu biodiesel through enzymatic hydroesterification. The complete hydrolysis oil using a 1:1 mass solution at 40 °C for 4 h 0.4% lipase from Thermomyces lanuginosus (TLL). Then, with use Eversa® Transform 2.0 in esterification step, statistical design used, varying temperature (25–55 °C), molar ratio between free fatty acids (FFAs) methanol (1:1 to 1:9), percentage biocatalyst (0.1% 0.9%), reaction time (1–5 h) Taguchi method. ideal levels obtained after treatment were 5 1:5 (FFAs/methanol) 0.9% biocatalyst. These optimal conditions validated by chromatographic analysis; following EN 14103 standard, sample showed an ester concentration 95.76%. evaluate stability Eversa FFAs. It observed molecular docking results that ligands interacted directly catalytic site. Through dynamics studies, it verified there no significant conformational changes studied complexes. Theoretical show feasibility this process.